Fusion of protoplasts of Bacillus megaterium.
نویسندگان
چکیده
منابع مشابه
Propionate induced lysis of protoplasts of Bacillus megaterium.
Previous studies (Storck and Wachsman, 1957; Weibull, Beckman, and Bergstrom, 1959; Wachsman, Fukuhara, and Nisman, 1960) on enzyme localization were performed with cell fractions, obtained by lysing protoplasts in media of low osmotic pressure. In an attempt to determine the effect of the tonicity of the lysing medium on the distribution of enzyme activities between the membrane containing and...
متن کاملReversion of Bacillus megaterium protoplasts to the bacillary form.
Photomicrographic evidence of reversion of Bacillus megaterium protoplasts to the bacillary form on soft agar plates hypertonic medium is demonstrated.
متن کاملGrowth and division of protoplasts of Bacillus megaterium and inhibition of division by penicillin.
Protoplasts of Bacillus megaterium grew well and divided in nutrient broth containing 0.5 m NaCl as the stabilizer. Protoplasts also grew when sucrose or succinate was used instead of NaCl; however, no division phenomena were observed. The sequence of growth and division was similar to the results obtained by McQuillen. Protoplasts enlarged from 1.8 to 3.5 mu in diameter, and then a small protu...
متن کاملCytological and Chemical Studies of the Growth of Protoplasts of Bacillus megaterium
Aeration of protoplasts of Bacillus megaterium in a succinate buffered nutrient broth led to marked growth similar to that already described by McQuillen, and the degree of chromatin synthesis in these growing forms prompted a combined cytological and chemical study. Growth was followed by phase contrast and by Feulgen stains, as well as by lipide phosphorus, nucleic acid, and protein analyses....
متن کاملMorphological study of the reversion to bacillary form, of Bacillus megaterium protoplasts.
Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) non oriented division of the protoplasts and (iii) outgrowth of the bacillary fo...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1976
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.73.6.2147